Increasing our understanding of biomolecular interactions through high resolution Atomic Force Microscopy
My research interests lie in understanding how variations in DNA structure can affect fundamental biological processes such as replication and transcription. To that end I am motivated by determining biomolecular mechanisms of action, with a long-term view to improved development of therapeutics. My scientific achievements include the first observation of single-molecule variations in the double-helical structure of DNA. I have been awarded both MRC and EPSRC fellowships to investigate the effect of DNA structure and topology on DNA-protein binding interactions.
I was lucky enough to have been selected to give a talk at the 2019 EMBO workshop on DNA topology and topoisomerases in genome dynamics in Les Diablerets. This was my second time attending this meeting, and it was particularly exciting for me, as I was returning to present data that came from a new collaboration with James Provan, Sean Colloms and Andrzej Stasiak which came from the last EMBO meeting.
AFMBiomed has marked some of the most important points in my academic career, so it seemed fitting that the first talk I gave since joining the Department of Materials Science and Engineering at Sheffield as a lecturer was at the 2019 AFMBiomed conference in beautiful Münster, expertly organised by Prof. Hermann Schillers.
Kavit attended the 8th Bio-AFM Summer School in Kanazawa, Japan, to investigate triplex DNA structure using super resolution in-liquid FM-AFM.
James Provan, from Sean Colloms’ lab at the University of Glasgow, visited our lab for a month, to carry out high resolution AFM imaging of DNA, kindly funded by the Microbiology Society. James wrote a blog post about his visit, which you can access by clicking on the title!
Congratulations to Dr Bennett on passing (and enjoying) her viva, and thank you to her brilliant examiners, Til Bachmann and Carmel Curtis for making it such a great experience. Its been an absolute pleasure working with Isabel for the past 5 years - I’m excited to see what the future has in store for you.
NAF 2019 was a brilliant meeting, organised by David Rueda, at the Royal Society of Chemistry. There were a series of brilliant talks, with an engaged and interested audience. Alice’s talk on supercoiling in DNA minicircles - ‘Untangling DNA, one molecule at a time’ - was well received, and she was awarded a model of the structure of the BDNA double helix, fabricated by Molecular Models. Thanks to the Nucleic Acids Group, David Rueda and his lab for organising an inspiring day of talks. We’re already looking forward to next year!
Our immune system relies on nanomachines, such as the membrane attack complex (MAC) to kill invasive bacteria in our blood. Our research, published in the EMBO journal and Nature Communications, provides us with a better understanding of how the immune system kills bacteria. This may guide the development of new therapies that harness the immune system against bacterial infections, and strategies that repurpose the immune system to act against other rogue cells in the body.
Great to meet so many prospective @msesheffield students today at the open day 😊
Walking to work thoughts. 75 hour weeks aren’t sustainable, anyone that says they are is not working effectively. A… https://t.co/cF6ixVkVJ0
This looks very interesting for any potential PhD students! https://t.co/UKO4OZqyph
Taking time out today to explore my new home by bike with my best friend. It’s incredible how unfit you can get in a month or two... #ouch
An E. coli bacterium with an outer membrane partially removed by the de-novo designed antimicrobial peptide Tilamin